4-Methylumbelliferyl phosphate

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4-甲基伞形基磷酸 (4-MUP) 是碱性磷酸酶的荧光底物。

4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate of alkaline phosphatases.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Serum acid phosphatase assay:1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0.2. Add the following agents (with the final concentration) 20 mM Sodium Tartrate (HY-128476), 15 mM Sodium Fluoride (HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM 1, 10-Phenanthroline (HY-W004544), 6 mM 8-Quinolinol (HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mM EDTA (HY-Y0682) and 6 mM EGTA (HY-D0861).3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5.4. Mix samples thoroughly and determine fluorescence by a fluorometer.

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4-MUP

Others

Other Targets

Others

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3368-04-5

256.15

C10H9O6P

>98% (HPLC)

H2O:20 mg/mL (78.07 mM; Need ultrasonic);

CC(c(ccc(OP(O)(O)=O)c1)c1O1)=CC1=O

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(-20℃)

With Ice Pack

≥ 2 years